:FixationStainingMounting leaf node



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URI https://neuroshapes.org/FixationStainingMounting
Label Fixation Staining Mounting
Description The Fixation Staining Mounting describes an activity in which brain tissue such as a brain slice is fixed (e.g. with formaldehyde), stained (e.g. using DAB staining) and mounted (onto an object slide for viewing with a microscope). The activity can follow e.g. a whole-cell patch clamp recording in which individual neurons are filled with biocytin. In the LNMC laboratory at EPFL, the fixation, staining and mounting procedure follows the protocol described here: dx.doi.org/10.17504/protocols.io.ujxeupn and dx.doi.org/10.17504/protocols.io.ujteunn.
SubClassOf (2) :ExperimentalActivityprov:Activity


PropertyDescriptionExpected TypeCardinality (min,max)
Properties from shape FixationStainingMountingShape
mountingMedia Mounting media is the medium that the sample is in while it is being imaged on the microscope such as Xylene or Mowiol. string (None---1)
used The collection of patched slices used SliceCollection (1---None)
stain Stain used for the detection of the cell labeling compound such as the chromogen DAB. string (None---1)
generated The collection of fixed and stained slices generated SliceCollection (1---None)
fixationMethod Method used for the fixation of the slice after electrophysiology such as PFA. string (None---1)
Properties from shape ActivityShape
used -- -- (1---None)
hadProtocol The value of nsg:hadProtocol should be of type nsg:Protocol. Protocol --

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@prefix nsg1: <https://bbp-nexus.epfl.ch/vocabs/bbp/neurosciencegraph/core/v0.1.0/> .
@prefix nxv: <https://bbp-nexus.epfl.ch/vocabs/nexus/core/terms/v0.1.0/> .
@prefix owl: <http://www.w3.org/2002/07/owl#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix provcommonshapes: <https://provshapes.org/commons/> .
@prefix provdatashapes: <https://provshapes.org/datashapes/> .
@prefix rdf: <http://www.w3.org/1999/02/22-rdf-syntax-ns#> .
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@prefix xml: <http://www.w3.org/XML/1998/namespace> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .

datashapes:fixationstainingmounting a ns1:Schema ;
    owl:imports commonshapes:activity ;
    prov:wasDerivedFrom "https://github.com/INCF/neuroshapes/blob/v0.3.15/modules/morphology/src/main/resources/schemas/neurosciencegraph/morphology/fixationstainingmounting/v1.0.0.json" ;
    ns1:shapes <https://neuroshapes.org/dash/fixationstainingmounting/shapes/FixationStainingMountingShape> .
{
  "@context": "https://incf.github.io/neuroshapes/contexts/schema.json",
  "@id": "nsg:dash/fixationstainingmounting",
  "@type": "nxv:Schema",
  "imports": [
    "nsg:commons/activity"
  ],
  "prov:wasDerivedFrom": "https://github.com/INCF/neuroshapes/blob/v0.3.15/modules/morphology/src/main/resources/schemas/neurosciencegraph/morphology/fixationstainingmounting/v1.0.0.json",
  "shapes": [
    "nsg:dash/fixationstainingmounting/shapes/FixationStainingMountingShape"
  ]
}

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